Multi-spectral imaging in mammalian cells using a home-built LSFM system

Aiswarya K. S.; Rinsa S. R.; Rahul Sharma; Srinivasa Murty Srinivasula; Mayanglambam Suheshkumar Singh

doi:10.1117/12.2649890

15 March 2023 Multi-spectral imaging in mammalian cells using a home-built LSFM system

Aiswarya K. S., Rinsa S. R., Rahul Sharma, Srinivasa Murty Srinivasula, Mayanglambam Suheshkumar Singh

Proceedings Volume 12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI; 123830L (2023) https://doi.org/10.1117/12.2649890
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Light sheet fluorescence microscopy (LSFM) works on fluorescence imaging, employing target-specific fluorescent proteins to label regions of interest in a sample. In this way, analyzing and manipulating information from different targets in a specimen requires labeling the specimen using multiple fluorophores. Multispectral imaging helps identify several target molecules simultaneously at once, thereby retrieving information from the entire volume of the sample. Here we report multispectral imaging studies on mammalian cells using our in-house built multispectral LSFM imaging system. Experimental studies were conducted on mammalian cells in alive conditions, and results are provided.

Citation Download Citation

Aiswarya K. S., Rinsa S. R., Rahul Sharma, Srinivasa Murty Srinivasula, and Mayanglambam Suheshkumar Singh "Multi-spectral imaging in mammalian cells using a home-built LSFM system", Proc. SPIE 12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI, 123830L (15 March 2023); https://doi.org/10.1117/12.2649890

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KEYWORDS

Multispectral imaging

Imaging systems

Image fusion

Fluorescence imaging

Biological imaging

Fluorophores

Laser sources

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