Label-free perioperative monitoring of monocytes and lymphocytes utilizing quantitative phase imaging with digital holographic microscopy

David Rene Steike; Michael Hessler; Burkhard Greve; Björn Kemper

doi:10.1117/12.2650523

16 March 2023 Label-free perioperative monitoring of monocytes and lymphocytes utilizing quantitative phase imaging with digital holographic microscopy

David Rene Steike, Michael Hessler, Burkhard Greve, Björn Kemper

Author Affiliations +

Proceedings Volume 12389, Quantitative Phase Imaging IX; 123890D (2023) https://doi.org/10.1117/12.2650523
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

In a prospective observational pilot study, we evaluated label-free quantitative phase imaging (QPI) with digital holographic microscopy (DHM) as a tool to describe changes in biophysical properties of lymphocytes and monocytes after cardiac surgery. The results of our study show the capability of DHM to quantify perioperative lymphocyte and monocyte alternations in cardiac surgery patients. The patterns of biophysical DHM data correlated with laboratory parameters, flow cytometric cell markers, and postoperative course. This exemplifies DHM as a promising tool to characterize and assess inflammatory processes and course of disease.

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Citation Download Citation

David Rene Steike, Michael Hessler, Burkhard Greve, and Björn Kemper "Label-free perioperative monitoring of monocytes and lymphocytes utilizing quantitative phase imaging with digital holographic microscopy", Proc. SPIE 12389, Quantitative Phase Imaging IX, 123890D (16 March 2023); https://doi.org/10.1117/12.2650523

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