3-D representation of microscopic specimens using confocal microscopy and digital image processing

Kjell Carlsson

doi:10.1117/12.19542

1 May 1990 3-D representation of microscopic specimens using confocal microscopy and digital image processing

Kjell Carlsson

Proceedings Volume 1245, Biomedical Image Processing; (1990) https://doi.org/10.1117/12.19542
Event: Electronic Imaging: Advanced Devices and Systems, 1990, Santa Clara, CA, United States

Abstract

In this paper a review is first given of how confocal fluorescence microscopy in combination with digital image processing can be utilized to present three-dimensional images of microscopic specimens. Examples of different display techniques are given as well as data on typical computing speeds when using a SUN 386i workstation. In the latter part of the paper we discuss signal quality and factors limiting the thickness of the specimen volume that can be studied. Multiple-wavelength registration is also described, as well as a method for eliminating detector "cross-talk" when recording two fluorophores with overlapping emission spectra.

Citation Download Citation

Kjell Carlsson "3-D representation of microscopic specimens using confocal microscopy and digital image processing", Proc. SPIE 1245, Biomedical Image Processing, (1 May 1990); https://doi.org/10.1117/12.19542

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