Optical system design of single laser flow cytometer

Guozhen Tan; Peijian Xiao; Haihuan Zhu; Meng Yuan; Ping Gong

doi:10.1117/12.2667510

6 March 2023 Optical system design of single laser flow cytometer

Guozhen Tan, Peijian Xiao, Haihuan Zhu, Meng Yuan, Ping Gong

Proceedings Volume 12553, Fourth International Conference on Optoelectronic Science and Materials (ICOSM 2022); 1255308 (2023) https://doi.org/10.1117/12.2667510
Event: 2022 4th International Conference on Optoelectronic Science and Materials (ICOSM2022), 2022, Henfei, China

Abstract

Optical system is the core of flow cytometry. According to the design requirements of flow cytometry, an optical system of flow cytometry with laser as light source is designed. According to the advantages and disadvantages of the laser source and the principle of scattering light and fluorescence, the laser wavelength is determined, and the optical path structure of laser shaping and scattering light and fluorescence detection is designed. The design results of the optical system are verified by using Zemax software simulation, point diagram, energy distribution detector and other tools. The results show that the spot size of the single laser flow cytometer optical system designed in this paper is less than 10 um×150 um after laser shaping, and the spot energy distribution is greater than 80%. The scattered light can be detected by the photodiode S10993-02 (Binsong) with the effective detection surface of 1 mm×1 mm; the fluorescence of 530 nm, 570 nm, 620 nm, 675 nm can be effectively detected by 3.7 mm×13 mm H9305-02 photomultiplier detector. The optical system of flow cytometer designed in this paper meets the basic requirements of flow cytometer detection, and provides reference for the subsequent flow cytometer system design.

Citation Download Citation

Guozhen Tan, Peijian Xiao, Haihuan Zhu, Meng Yuan, and Ping Gong "Optical system design of single laser flow cytometer", Proc. SPIE 12553, Fourth International Conference on Optoelectronic Science and Materials (ICOSM 2022), 1255308 (6 March 2023); https://doi.org/10.1117/12.2667510

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available