Functionalization of nanostructured surfaces for optical sensor platforms

Vicente Silva Mattos; Jarbas Caiado de Castro Neto; Francisco Eduardo Gontijo Guimarães; Raphael Antonio Caface; Elsa M. Materón

doi:10.1117/12.2664866

31 May 2023 Functionalization of nanostructured surfaces for optical sensor platforms

Vicente Silva Mattos, Jarbas Caiado de Castro Neto, Francisco Eduardo Gontijo Guimarães, Raphael Antonio Caface, Elsa M. Materón

Author Affiliations +

Proceedings Volume 12572, Optical Sensors 2023; 1257212 (2023) https://doi.org/10.1117/12.2664866
Event: SPIE Optics + Optoelectronics, 2023, Prague, Czech Republic

Conference Poster

Abstract

In every field of science, new techniques for target molecule detection are increasingly required. This work presents itself as a method for developing new sensors that can detect target molecules even in samples with low concentrations. The aim of the current research project is to create an optical sensor substrate that is precise and specific for fluorescent or fluorescently marked targets. We produced nanostructured surfaces on bulk silver substrates using a femtosecond pulsed laser (Laser Libra Ti:Saphira from Coherent). These surfaces have a larger superficial area, which increases the fluorescence of molecules nearby by due to the surface plasmon resonance, in the effect of metal-enhanced fluorescence (MEF). The COVID virus spike antibody (SARS-CoV-2(2019-nCoV)) and a secondary fluorescently marked antibody (Alexa Fluor™ 633 goat anti-human IgG (H+L)) was used to functionalize the nanostructures. Initial results indicated that the functionalization process was successful in achieving our initial goals, presenting the proposal's methods as an effective route for the development of biosensors. The primary antibody was initially detected in a very low concentration (0.0525 ng/uL), and the fluorescent signal was enhanced on the nanostructured portion of the surface by 6.3 times more than it was on the surface without modification.

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Vicente Silva Mattos, Jarbas Caiado de Castro Neto, Francisco Eduardo Gontijo Guimarães, Raphael Antonio Caface, and Elsa M. Materón "Functionalization of nanostructured surfaces for optical sensor platforms", Proc. SPIE 12572, Optical Sensors 2023, 1257212 (31 May 2023); https://doi.org/10.1117/12.2664866

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