Design of fluorescence sensor for detecting GPC3 based on gold nanocluster-graphene oxide composite system

Wei Chen; Xiaoqing Yao; Lin Lu; Guiyin Li

doi:10.1117/12.2674166

9 March 2023 Design of fluorescence sensor for detecting GPC3 based on gold nanocluster-graphene oxide composite system

Wei Chen, Xiaoqing Yao, Lin Lu, Guiyin Li

Proceedings Volume 12600, International Conference on Optoelectronic Materials and Devices (ICOMD 2022); 126001C (2023) https://doi.org/10.1117/12.2674166
Event: 2022 International Conference on Optoelectronic Materials and Devices, 2022, Chongqing, China

Abstract

Phosphatidylinositol proteoglycan-3 (GPC3) as an oncoprotein is a potential novel marker for hepatocellular carcinoma (HCC). In this paper, a GPC3 fluorescence sensor was constructed based on the fluorescence resonance energy transfer (FRET) interaction between graphene oxide (GO) and gold nanoclusters (Au NCs) with GPC3 as the target. In the solution system, as GO and Au NCs-GPC3_Apt approach each other, the fluorescence of Au NCs-GPC3_Apt was quenched, and the fluorescence of the whole solution system is weakened. When adding GPC3 into the whole solution, the fluorescence intensity in Au NCs-GPC3_Apt/GO FRET system can be increased. The amount of GPC3 was inferred based on the degree of fluorescence recovery of the solution system. In the range of 5~100 ng/mL, the fluorescence recovery rate was linearly related to the concentration of GPC3 with a correlation coefficient of 0.99645. It is proved that this GPC3 fluorescence sensor has outstanding sensitivity, stability and has great potential in the early diagnosis of cancer.

Citation Download Citation

Wei Chen, Xiaoqing Yao, Lin Lu, and Guiyin Li "Design of fluorescence sensor for detecting GPC3 based on gold nanocluster-graphene oxide composite system", Proc. SPIE 12600, International Conference on Optoelectronic Materials and Devices (ICOMD 2022), 126001C (9 March 2023); https://doi.org/10.1117/12.2674166

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