Two-photon laser polymerized microlenses for non-linear excitation microscopy of biological samples

A. Nardini; R. Martínez Vázquez; M. Marini; C. Conci; M. Bouzin; M. Collini; R. Osellame; G. Cerullo; B. S. Kariman; M. Farsari; E. Kabouraki; G. Chirico; M. T. Raimondi

doi:10.1117/12.2672894

11 August 2023 Two-photon laser polymerized microlenses for non-linear excitation microscopy of biological samples

A. Nardini, R. Martínez Vázquez, M. Marini, C. Conci, M. Bouzin, M. Collini, R. Osellame, G. Cerullo, B. S. Kariman, M. Farsari, E. Kabouraki, G. Chirico, M. T. Raimondi

Author Affiliations +

Proceedings Volume 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI; 1262206 (2023) https://doi.org/10.1117/12.2672894
Event: SPIE Optical Metrology, 2023, Munich, Germany

Abstract

We report new methods of two-photon polymerization of microlenses with high numerical aperture, large diameter and good optical quality. We characterize the aberrations of these lenses that, coupled to raster scanning optical microscopes, allow two-photon excitation imaging of cells. In-vivo non-linear imaging will be also discussed, opening the possibility to use these micro-lenses in implants for the continuous inspection of biological dynamics in vivo.

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A. Nardini, R. Martínez Vázquez, M. Marini, C. Conci, M. Bouzin, M. Collini, R. Osellame, G. Cerullo, B. S. Kariman, M. Farsari, E. Kabouraki, G. Chirico, and M. T. Raimondi "Two-photon laser polymerized microlenses for non-linear excitation microscopy of biological samples", Proc. SPIE 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, 1262206 (11 August 2023); https://doi.org/10.1117/12.2672894

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