Enhancing the axial resolution of an optoacoustic  microscopy imaging instrument by using a pico-second pulse duration laser

Gianni Nteroli; Giulia Messa; Stella Koutsikou; Manoj K. Dasa; Antti Penttinen; Antti Härkönen; Mircea Guina; Adrian Podoleanu; Adrian Bradu

doi:10.1117/12.2670901

11 August 2023 Enhancing the axial resolution of an optoacoustic microscopy imaging instrument by using a pico-second pulse duration laser

Gianni Nteroli, Giulia Messa, Stella Koutsikou, Manoj K. Dasa, Antti Penttinen, Antti Härkönen, Mircea Guina, Adrian Podoleanu, Adrian Bradu

Author Affiliations +

Proceedings Volume 12631, Opto-Acoustic Methods and Applications in Biophotonics VI; 1263106 (2023) https://doi.org/10.1117/12.2670901
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

In conventional optoacoustic microscopy, nanosecond pulse duration lasers are employed. When a laser delivering shorter pulse durations is used, it is expected that, from a theoretical point of view, broader, higher-frequency acoustic waves to be generated, therefore a better axial resolution of the instrument. In the present report, this advantage, offered by a picosecond duration pulse laser, to experimentally demonstrate that the axial resolution of an optoacoustic microscopy instrument can be enhanced was exploited. In comparison to a 2 ns pulse duration, an improvement in the axial resolution of ~50% is demonstrated by using excitations with pulses of duration ⪅100 ps. Details of an optoacoustic microscopy instrument, operating at 532 nm, capable to provide high-resolution axial and lateral optoacoustic images, are also presented. The capabilities of the instrument are demonstrated by in-vivo images of Xenopus laevis brain with a similar ~ 3.8 μm lateral resolution throughout the whole axial imaging range.

Citation Download Citation

Gianni Nteroli, Giulia Messa, Stella Koutsikou, Manoj K. Dasa, Antti Penttinen, Antti Härkönen, Mircea Guina, Adrian Podoleanu, and Adrian Bradu "Enhancing the axial resolution of an optoacoustic microscopy imaging instrument by using a pico-second pulse duration laser", Proc. SPIE 12631, Opto-Acoustic Methods and Applications in Biophotonics VI, 1263106 (11 August 2023); https://doi.org/10.1117/12.2670901

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
3 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Optoacoustics

Equipment

Microscopy

Pulsed laser operation

Image resolution

Resolution enhancement technologies

Picosecond phenomena

Show All Keywords

Keywords/Phrases

Search In:

Publication Years