Fiber optic nanomechanical probe for single-cell mechanics analysis

Changrui Liao; Mengqiang Zou; Lei Xu; Ying Wang; Dejun Liu; Yiping Wang

doi:10.1117/12.2678308

23 May 2023 Fiber optic nanomechanical probe for single-cell mechanics analysis

Changrui Liao, Mengqiang Zou, Lei Xu, Ying Wang, Dejun Liu, Yiping Wang

Proceedings Volume 12643, European Workshop on Optical Fibre Sensors (EWOFS 2023); 126431Q (2023) https://doi.org/10.1117/12.2678308
Event: European Workshop on Optical Fibre Sensors (EWOFS 2023), 2023, Mons, Belgium

Abstract

Ultrasensitive nanomechanical instruments, e.g., atomic force microscopy (AFM), can be used to perform delicate biomechanical measurements and reveal the complex mechanical environment of biological processes. However, these instruments are limited because of their size and complex feedback system. Here, we demonstrate a miniature fiber optical nanomechanical probe (FONP) that can be used to detect the mechanical properties of single cells. The stiffness matching of the FONP and sample can be realized by customizing the microcantilever’s spring constant. As a proof-of concept, three FONPs with spring constants varying from 0.421 N/m to 52.6 N/m by more than two orders of magnitude were prepared. The Young's modulus of heterogeneous soft materials, such as polydimethylsiloxane, onion cells and MCF-7 cells, were successfully measured. FONP has made substantial progress in realizing basic biological discoveries, and our strategy provides a universal protocol for directly programming fiber-optic AFMs.

Citation Download Citation

Changrui Liao, Mengqiang Zou, Lei Xu, Ying Wang, Dejun Liu, and Yiping Wang "Fiber optic nanomechanical probe for single-cell mechanics analysis", Proc. SPIE 12643, European Workshop on Optical Fibre Sensors (EWOFS 2023), 126431Q (23 May 2023); https://doi.org/10.1117/12.2678308

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