Using flat-top light sheet generated by femtosecond-pulsed laser for optical manipulation of microscopic particles

Krishangi Krishna; Joshua A. Burrow; Mbaye Diouf; Jieliyue Sun; Mitchell Harling; Kimani C. Toussaint Jr.

doi:10.1117/12.2678364

5 October 2023 Using flat-top light sheet generated by femtosecond-pulsed laser for optical manipulation of microscopic particles

Krishangi Krishna, Joshua A. Burrow, Mbaye Diouf, Jieliyue Sun, Mitchell Harling, Kimani C. Toussaint Jr.

Author Affiliations +

Proceedings Volume 12649, Optical Trapping and Optical Micromanipulation XX; 126490L (2023) https://doi.org/10.1117/12.2678364
Event: SPIE Nanoscience + Engineering, 2023, San Diego, California, United States

Abstract

Optical tweezers (OT) has proven to be an indispensable tool for elucidating phenomena in colloidal physics and for biomedical applications. Additionally, OT has been used to apply sub-piconewton forces on microscopic particles, for example in cells, as well as to measure displacements with nanometer resolution to extrapolate mechanical properties. Recently, an OT platform based on light sheet microscopy with a continuous wave laser has been developed to trap microscopic dielectric particles. However, the reduced gradient force resulting from the light sheet intensity distribution produces a trap stiffness an order of magnitude lower than its traditional circularly symmetric Gaussian counterpart. As a result, a high laser power, on the order of 50 mW is required, which risks phototoxicity for biological applications. In this work, we first compare the trap stiffnesses of continuous wave and femtosecond pulsed laser sources on dielectric particles in sub-1 mW scale. Next, we demonstrate the OT of dielectric spheres using a flat-top light sheet generated by a femtosecond pulsed laser source utilizing average powers as low as 1 mW. We propose leveraging flat-top light sheet OT to characterize the local and average mechanical properties of biological specimens.

Conference Presentation

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Krishangi Krishna, Joshua A. Burrow, Mbaye Diouf, Jieliyue Sun, Mitchell Harling, and Kimani C. Toussaint Jr. "Using flat-top light sheet generated by femtosecond-pulsed laser for optical manipulation of microscopic particles", Proc. SPIE 12649, Optical Trapping and Optical Micromanipulation XX, 126490L (5 October 2023); https://doi.org/10.1117/12.2678364

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