Femtosecond laser rapidly targets gene transfection and regulates cell expression

Ziyan Song; Yanhong Hua; Qian Qian; Baoshan Guo

doi:10.1117/12.2690198

17 August 2023 Femtosecond laser rapidly targets gene transfection and regulates cell expression

Ziyan Song, Yanhong Hua, Qian Qian, Baoshan Guo

Proceedings Volume 12757, 3rd International Conference on Laser, Optics, and Optoelectronic Technology (LOPET 2023); 1275724 (2023) https://doi.org/10.1117/12.2690198
Event: 3rd International Conference on Laser, Optics and Optoelectronic Technology (LOPET 2023), 2023, Kunming, China

Abstract

Genetic engineering should be achieved by means of gene transfection methods, but traditional gene transfection technology has many disadvantages such as high cell lethality, easy introduction of pollutants, high cost, etc., while femtosecond laser has ultra-short single pulse time, ultra-high peak power, and small ablation area, which is in line with the requirements of cell transfection. In this paper, based on gene transfection and regulation of cell expression, combined with the advantages of femtosecond laser ultra-fast and super strong, femtosecond laser transfection was used to transfect protein-sensitized functional proteins into embryonic cell lines, and the influence of various processing parameters on transfection efficiency results was studied, high transfection efficiency and better transfection expression results were obtained, high-precision and efficient transfection results were realized, and the mechanism of laser-cell interaction was established, so as to realize laser precision regulation of cell transfection.

Citation Download Citation

Ziyan Song, Yanhong Hua, Qian Qian, and Baoshan Guo "Femtosecond laser rapidly targets gene transfection and regulates cell expression", Proc. SPIE 12757, 3rd International Conference on Laser, Optics, and Optoelectronic Technology (LOPET 2023), 1275724 (17 August 2023); https://doi.org/10.1117/12.2690198

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