Paper
27 November 2023 Modular digital holographic microscopy
Zhengyuan Tang, Bryan M. Hennelly
Author Affiliations +
Abstract
This paper reviews recent advancements in digital holographic microscopy, whereby a simple and inexpensive module can be added to an existing life science microscope to imbue it with quantitative phase capabilities while leveraging on the utility of pre-existing functionalities such as fluorescence imaging. Common-path interferometry is one such approach, whereby both the reference and object wavefield propagate through the same optical elements. Other approaches employ beamsplitters to separate the paths. A key feature of many of these both cases is the principle of self-referencing, whereby a clean reference plane wave can be produced from a copy of the object wavefield. As well as review the basic principles of digital holographic microscopy in general, we review the various variants of modular digital holographic microscopy that have been proposed over the past decade and offer insights into their performance and limitations.
(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.
Zhengyuan Tang and Bryan M. Hennelly "Modular digital holographic microscopy", Proc. SPIE 12768, Holography, Diffractive Optics, and Applications XIII, 1276815 (27 November 2023); https://doi.org/10.1117/12.2689214
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KEYWORDS
Diffraction

Optical tomography

Phase imaging

Microscopes

Design and modelling

Digital holography

Holography

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