The single cell sorting technique is important for proper treatment strategies and prognosis. In this work, we develop a system to real-time measure the Mueller matrix of multi-angle polarization scattering from an individual cell. The method measures the Mueller matrix of polarization scattering at two different angles, 60° and 120°, and can effectively analyze the microstructure, morphology and complex refractive index of single cells. The principal advantage of the method is that the multi-dimensional polarization parameters can improve the accuracy of cell sorting and quantitative analysis. Our phantom experimental results validate the advantages of multi-angle polarization scattering measurements. And then, Experiments on subtype classification of Acute lymphoblastic leukemia (ALL) support the validity and feasibility of the method, indicating that the Mueller matrix of multiple polarization scattering angles have important potential applications in high-throughput cell sorting.
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