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13 March 2024 Comparison of continuous and pulsed optogenetic stimulation for embryonic cardiodynamic studies
Guzel R. Musina, Deirdre M. Scully, Andrew L. Lopez III, Irina V. Larina
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Proceedings Volume 12819, Diagnostic and Therapeutic Applications of Light in Cardiology 2024; 128190L (2024) https://doi.org/10.1117/12.3005599
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
The goal of this study is to explore the potential of pulsed and continuous wave light optogenetic stimulation on mouse embryonic cardiodynamics. Toward this goal, we engineered mouse embryos expressing the light-sensitive protein Channelrhodopsin-2 (ChR2) ubiquitously through the embryo. The embryos were dissected live and the optogenetic light stimulation of ChR2 at 473-nm was performed under imaging guidance. The pulsed stimulation allowed for a range of cardiodynamic behaviors and was overall found to have a milder effect on embryo viability, while the continuous wave stimulation provided an advantage in the faster mapping of optogenetic cardiac responses.
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Guzel R. Musina, Deirdre M. Scully, Andrew L. Lopez III, and Irina V. Larina "Comparison of continuous and pulsed optogenetic stimulation for embryonic cardiodynamic studies", Proc. SPIE 12819, Diagnostic and Therapeutic Applications of Light in Cardiology 2024, 128190L (13 March 2024); https://doi.org/10.1117/12.3005599
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KEYWORDS
Optogenetics
Animal model studies
Biomechanics
Heart
Proteins
Animals
Diagnostics
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