Imaging zebrafish embryonic development with multimodal optical coherence tomography and light-sheet fluorescence microscopy

Md Mobarak Karim; Ruijiao Sun; Oscar E. Ruiz; Leah A. Lewis; Manmohan Singh; Harshdeep S. Chawla; Alexander W. Schill; David Mayerich; Joshua D. Wythe; Kirill V. Larin

doi:10.1117/12.3001234

12 March 2024 Imaging zebrafish embryonic development with multimodal optical coherence tomography and light-sheet fluorescence microscopy

Md Mobarak Karim, Ruijiao Sun, Oscar E. Ruiz, Leah A. Lewis, Manmohan Singh, Harshdeep S. Chawla, Alexander W. Schill, David Mayerich, Joshua D. Wythe, Kirill V. Larin

Author Affiliations +

Proceedings Volume 12834, Multimodal Biomedical Imaging XIX; 1283402 (2024) https://doi.org/10.1117/12.3001234
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Zebrafish are a widely used developmental model because of their transparent embryos and external development. These distinctive characteristics provide valuable insights into embryonic development. Optical coherence tomography (OCT) offers label-free structural imaging and has emerged as a preferred tool for embryonic imaging. On the other hand, light sheet fluorescence microscopy (LSFM) enables time-lapse molecular imaging of multi-hour to multi-day developmental processes due to its low phototoxicity and photobleaching compared to traditional confocal fluorescence microscopy. We developed a multimodal imaging system to obtain concurrent structural and molecular information by combining OCT and LSFM for embryonic imaging. A Michelson-type swept-source OCT system with a central wavelength of 1050 nm, the bandwidth of 100 nm, and sweep rate of 100 kHz captured the structural information with a lateral resolution of ~15 μm and an axial resolution of ~7 μm. The LSFM system captured the molecular information with a transverse resolution of ~2.1 μm and an axial resolution of ~13 μm. The optically co-aligned OCT and LSFM beams were scanned through the same scan head for trivial co-registration of the multimodal images. We imaged 1-5 μm green fluorescence microbeads to show the capability of this system. We then conducted imaging of zebrafish vasculature development with a transgenic line, Tg(kdrl:EGFP), where the erythroblasts express GFP. The results show that the multimodal system enables us to provide co-registered zebrafish structural and functional imaging.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Md Mobarak Karim, Ruijiao Sun, Oscar E. Ruiz, Leah A. Lewis, Manmohan Singh, Harshdeep S. Chawla, Alexander W. Schill, David Mayerich, Joshua D. Wythe, and Kirill V. Larin "Imaging zebrafish embryonic development with multimodal optical coherence tomography and light-sheet fluorescence microscopy", Proc. SPIE 12834, Multimodal Biomedical Imaging XIX, 1283402 (12 March 2024); https://doi.org/10.1117/12.3001234

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available