Direct blood cell flow imaging in microvascular networks

Gyounghwan Kim; Hyun-Sang Park; Paul Shin; Taeguk Eom; Jin-Hui Yoon; Yong Jeong; Wang-Yuhl Oh

doi:10.1117/12.3001459

12 March 2024 Direct blood cell flow imaging in microvascular networks

Gyounghwan Kim, Hyun-Sang Park, Paul Shin, Taeguk Eom, Jin-Hui Yoon, Yong Jeong, Wang-Yuhl Oh

Proceedings Volume 12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; 1284804 (2024) https://doi.org/10.1117/12.3001459
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Blood flow dynamics in microvascular networks are intimately related to the health of tissues and organs. While numerous imaging modalities and techniques have been developed to assess blood flow dynamics for various applications, their utilization has been hampered by limited imaging speed and indirect quantification of blood flow dynamics. Here, we demonstrate direct blood cell flow imaging (DBFI) that provides visualization of individual motions of blood cells over a field of 0.71 mm x 1.42 mm with a time resolution of 0.69 ms (1450 frames per second) without using any exogenous agents. DBFI enables precise dynamic analysis of blood cell flow velocities and fluxes in various vessels over a large field, from capillaries to arteries and veins, with unprecedented time resolution. Several exemplary applications of DBFI illustrate the potential of this new imaging technology.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Gyounghwan Kim, Hyun-Sang Park, Paul Shin, Taeguk Eom, Jin-Hui Yoon, Yong Jeong, and Wang-Yuhl Oh "Direct blood cell flow imaging in microvascular networks", Proc. SPIE 12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, 1284804 (12 March 2024); https://doi.org/10.1117/12.3001459

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