A miniaturized test for stress biomarker detection by quantum dots on microarrays

Anusha Kishore; Arun Mathew Varughese; Bernhard Roth; Carsten Zeilinger

doi:10.1117/12.3002242

27 March 2024 A miniaturized test for stress biomarker detection by quantum dots on microarrays

Anusha Kishore, Arun Mathew Varughese, Bernhard Roth, Carsten Zeilinger

Proceedings Volume 12850, Optical Diagnostics and Sensing XXIV: Toward Point-of-Care Diagnostics; 1285006 (2024) https://doi.org/10.1117/12.3002242
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We aim to develop a benchtop imaging device to quantitatively measure stress biomarkers like HSP90 on a biochip. Measurement of HSP90 is based on the masking of fluorescence of Streptavidin conjugated Quantum dots (Sav-QDs) when HSP90 attaches to it. The higher the masking of fluorescence, the higher the HSP90 concentration. Measurement of fluorescence is done by a custom-built optical device. The goal in this work was to demonstrate a testing system which is handy to use in the field, cheaper and with a simplified readout system for the users. Sav-QDs are fixed on nitrocellulose coated glass slides (NC slides). A biotinylated antibody is attached to Sav-QD. When HSP90 interacts with the antibody it causes masking of fluorescence, which is dependent on the concentration of HSP90 in serum. The imaging device developed for measurement is based on a CMOS sensor. It uses narrow bandpass filters, optically eliminating fluorescence produced by the background. Analysis of the results shows that these agree well with those of standard laboratory equipment using a photomultiplier tube (PMT) scanner to detect HSP90 in the nanomolar range. Results from the work show that the used approach is promising for developing a multifunctional, robust, and point-of-care detection system for stress biomarkers.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Anusha Kishore, Arun Mathew Varughese, Bernhard Roth, and Carsten Zeilinger "A miniaturized test for stress biomarker detection by quantum dots on microarrays", Proc. SPIE 12850, Optical Diagnostics and Sensing XXIV: Toward Point-of-Care Diagnostics, 1285006 (27 March 2024); https://doi.org/10.1117/12.3002242

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