Video-rate spontaneous Raman imaging

Lochlannn Dunn; Haokun Luo; Nava R. Subedi; Ramachandran Kasu; Armando G. McDonald; Demetrios N. Christodoulides; Andreas E. Vasdekis

doi:10.1117/12.3003021

12 March 2024 Video-rate spontaneous Raman imaging

Lochlannn Dunn, Haokun Luo, Nava R. Subedi, Ramachandran Kasu, Armando G. McDonald, Demetrios N. Christodoulides, Andreas E. Vasdekis

Author Affiliations +

Proceedings Volume 12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; 128540K (2024) https://doi.org/10.1117/12.3003021
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We demonstrate video-rate spontaneous Raman imaging by combining lightsheet microscopy that harnesses non-diffracting Airy beams to efficiently illuminate large specimen regions with image acquisition and reconstruction at the subphoton per pixel levels. We validated these benefits by imaging a wide variety of samples, including organic materials and the metabolic activity of single living yeast cells. Overall, our method not only enables video-rate imaging rates, but also requires 1000-fold less irradiance levels than state-of-the-art coherent Raman microscopy. As such, we expect this approach will greatly accelerate the reliability and reproducibility of Raman imaging in both fundamental research and clinical applications.

Conference Presentation

Citation Download Citation

Lochlannn Dunn, Haokun Luo, Nava R. Subedi, Ramachandran Kasu, Armando G. McDonald, Demetrios N. Christodoulides, and Andreas E. Vasdekis "Video-rate spontaneous Raman imaging", Proc. SPIE 12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, 128540K (12 March 2024); https://doi.org/10.1117/12.3003021

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available