Laser micromachining for enhancing lateral flow assay colorimetric signal sensitivity

Gazy Albedry; Mohamed Ahmed Baba; Martynas Simanavičius; Laimis Silimavičius; Gintautas Gylys; Tomas Tamulevičius

doi:10.1117/12.3012595

20 June 2024 Laser micromachining for enhancing lateral flow assay colorimetric signal sensitivity

Gazy Albedry, Mohamed Ahmed Baba, Martynas Simanavičius, Laimis Silimavičius, Gintautas Gylys, Tomas Tamulevičius

Proceedings Volume 13008, Biophotonics in Point-of-Care III; 130080M (2024) https://doi.org/10.1117/12.3012595
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Conference Poster

Abstract

Although lateral flow assays (LFAs) are currently being a handful of diagnostic technologies that can identify severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other common respiratory viruses in one strip, it remains a grand challenge to substantially enhance their sensitivity. We propose here a straightforward method to overcome such drawbacks by employing nitrocellulose (NC) membrane femtosecond laser micromachining to control the analyte flow rate. The findings provided in this work indicate that tailoring the diameters of the μ-channels in NC can effectively expedite the immunological reaction time between the analyte and the labeled antibody, leading to an observable signal increase compared to pristine LFA.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Gazy Albedry, Mohamed Ahmed Baba, Martynas Simanavičius, Laimis Silimavičius, Gintautas Gylys, and Tomas Tamulevičius "Laser micromachining for enhancing lateral flow assay colorimetric signal sensitivity", Proc. SPIE 13008, Biophotonics in Point-of-Care III, 130080M (20 June 2024); https://doi.org/10.1117/12.3012595

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