CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 13139 > Article
Presentation
8 October 2024 Super-resolution spectral chemical imaging to study metabolic correlation in subcellular organelle
Hongje Jang, Yajuan Li, Zhi Li, Lingyan Shi
Author Affiliations +
Proceedings Volume 13139, Ultrafast Nonlinear Imaging and Spectroscopy XII; 1313908 (2024) https://doi.org/10.1117/12.3028288
Event: Optical Engineering + Applications, 2024, San Diego, California, United States
Abstract
Investigating metabolic reactions within cells is crucial for unraveling the numerous biological functions. Established imaging modalities, including MRI, PET, Fluorescence, and Mass Spectrometry, present various drawbacks. To address these issues, we have developed a nonlinear multimodal imaging system. This system combines stimulated Raman scattering, multiphoton fluorescence, and second harmonic generation. It was designed to probe the spatial distribution of metabolic activities within cells and tissues by measuring multiple molecular signals. To support the analysis scheme, we have also pioneered cutting-edge algorithms, notably the Adam-based Pointillism Deconvolution (A-PoD) and Correlation Coefficient Mapping (CoCoMap). They allow us to analyze correlations between super-resolution images of nanoscale Regions of Interest. Additionally, our research has introduced a novel clustering algorithm known as Multi-SRS reference matching (Multi-SRM). This algorithm is particularly tailored to isolate signals exclusively from specific subcellular organelles. The application of this innovation offers significant potential to study aging and disease related metabolic changes.
Conference Presentation
© (2024) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Hongje Jang, Yajuan Li, Zhi Li, and Lingyan Shi "Super-resolution spectral chemical imaging to study metabolic correlation in subcellular organelle", Proc. SPIE 13139, Ultrafast Nonlinear Imaging and Spectroscopy XII, 1313908 (8 October 2024); https://doi.org/10.1117/12.3028288
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
;
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Super resolution
Imaging spectroscopy
Multimodal imaging
Second harmonic generation
Tissues
Visualization
Molecular interactions
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top