Electrophoretic mobility and conformational changes of DNA in agarose gels

Benjamin Chu; Zhulun Wang

doi:10.1117/12.57294

1 May 1991 Electrophoretic mobility and conformational changes of DNA in agarose gels

Benjamin Chu, Zhulun Wang

Proceedings Volume 1403, Laser Applications in Life Sciences; (1991) https://doi.org/10.1117/12.57294
Event: Laser Applications in Life Sciences, 1990, Moscow, Russian Federation

Abstract

We present observations of time-dependent electrophoretic mobility and conformational changes of a polyelectrolyte DNA in agarose gels under an applied electric field. The short-time averaged electrophoretic mobility can be measured by means of movement of fluorescence pattern after photobleaching (MOFPAP) while the average conformational change of an assembly of polyelectrolyte chains can be revealed by means of transient electric birefringence (TEB) which measures the rise and fall of the time-dependent optical anisotropy of the polyelectrolyte chains under an applied electric field. The results from a combination of the two techniques (MOFPAP and TEB) are discussed in terms of its implications on the mechanisms responsible for pulsed-field gel electrophoresis. 1.

Citation Download Citation

Benjamin Chu and Zhulun Wang "Electrophoretic mobility and conformational changes of DNA in agarose gels", Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); https://doi.org/10.1117/12.57294

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available