Photon correlation spectroscopy of chromaffin granules lysis

Yu. A. Ermakov; J. Engel; E. Donath

doi:10.1117/12.57390

1 May 1991 Photon correlation spectroscopy of chromaffin granules lysis

Yu. A. Ermakov, J. Engel, E. Donath

Proceedings Volume 1403, Laser Applications in Life Sciences; (1991) https://doi.org/10.1117/12.57390
Event: Laser Applications in Life Sciences, 1990, Moscow, Russian Federation

Abstract

When bovine chromaffin granules are incubated in hyperosmotic sucrose solutions and subsequently transfered back towards isoosmolarity they undergo lysis ("hyperosmotic relaxation lysis"). The structural basis for this type of lysis, and the generally encountered hypotonic lysis were studied by photon correlation spectroscopy (PCS) and freeze-fracture electron microscopy (EM). Hyperosmotically pretreated granules showed no significant difference in size as measured by PCS compared with the control. Under the same conditions EM showed the formation of "twins" and "triplets" and intragranular vesicles of 50-200 nm in diameter.' We suggest that hyperosmotic relaxation lysis and to a certain extent the hypoosmotic one are caused by the adhesion of the intragranular vesicles to the outer membrane preventing the granule membrane from reexpanding and thus leading to its rupture.

Citation Download Citation

Yu. A. Ermakov, J. Engel, and E. Donath "Photon correlation spectroscopy of chromaffin granules lysis", Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); https://doi.org/10.1117/12.57390

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