Direct measurement of vibrational energy relaxation in photoexcited deoxyhemoglobin using picosecond Raman spectroscopy

John B. Hopkins; Xiaobing Xu; Robert Lingle Jr.; Huiping Zhu; Soo-chang Yu

doi:10.1117/12.44224

1 June 1991 Direct measurement of vibrational energy relaxation in photoexcited deoxyhemoglobin using picosecond Raman spectroscopy

John B. Hopkins, Xiaobing Xu, Robert Lingle Jr., Huiping Zhu, Soo-chang Yu

Author Affiliations +

Proceedings Volume 1432, Biomolecular Spectroscopy II; (1991) https://doi.org/10.1117/12.44224
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

We have directly observed hot vibrations in photoexcited deoxyhemoglobin. The data quantitatively indicate a heme vibrational temperature of 20 K above room temperature within the time sampled by an 8ps laser pulse. The slow component of vibrational relaxation occurs with a rate constant of (2.5ps)1. Similar dynamics are observed in oxyhemoglobin. The initial stages of recombmation of 02 with the heme following photodissociation of oxyhemoglobin are observed in the transient vibrational spectrum. Contrary to previous work, we do not observe the influence of protein dynamics on 02 recombination.

Citation Download Citation

John B. Hopkins, Xiaobing Xu, Robert Lingle Jr., Huiping Zhu, and Soo-chang Yu "Direct measurement of vibrational energy relaxation in photoexcited deoxyhemoglobin using picosecond Raman spectroscopy", Proc. SPIE 1432, Biomolecular Spectroscopy II, (1 June 1991); https://doi.org/10.1117/12.44224

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