Automated immunofluorescence measurement system using a bleaching method

Yanmin Tao; G. Boeck; Norbert H. Nessler; Erwin Hochmair

doi:10.1117/12.58280

1 April 1992 Automated immunofluorescence measurement system using a bleaching method

Yanmin Tao, G. Boeck, Norbert H. Nessler, Erwin Hochmair

Proceedings Volume 1640, Time-Resolved Laser Spectroscopy in Biochemistry III; (1992) https://doi.org/10.1117/12.58280
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

The bleaching process, which is the fading of the fluorescence of dye molecules under continuous excitation, can be used to analyze the specificity of the antibody-antigen reaction in immunological tests. The automated immunofluorescence measurement system based on the analysis of bleaching characteristics, which can be described by the formfactor beside the maximum fluorescence intensity, has been developed. These two parameters can be used in fluorescence imaging to detect rare events among nonspecific staining.

Citation Download Citation

Yanmin Tao, G. Boeck, Norbert H. Nessler, and Erwin Hochmair "Automated immunofluorescence measurement system using a bleaching method", Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); https://doi.org/10.1117/12.58280

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