Submicron optical sources for single macromolecule detection

Robert J. G. Carr; Sumaia Al-Shukri; Elaine A. Perkins; Thierry Rodard; Tony Atkinson

doi:10.1117/12.143509

30 April 1993 Submicron optical sources for single macromolecule detection

Robert J. G. Carr, Sumaia Al-Shukri, Elaine A. Perkins, Thierry Rodard, Tony Atkinson

Proceedings Volume 1796, Chemical, Biochemical, and Environmental Fiber Sensors IV; (1993) https://doi.org/10.1117/12.143509
Event: Fibers '92, 1992, Boston, MA, United States

Abstract

Nanobit holographic techniques, such as high-energy electron beam lithography, allow apertures of < 50 nm diam. to be fabricated in the metal coating of SPR sensor devices. When the SPR slab waveguide is edge illuminated with a laser, the apertures act as intense scattering centers of the underlying radiation, the scattered light from which is readily detectable by conventional optical microscopy. Given the dimensions of these sub-wavelength optical sources are within an order of magnitude of those of individual biological macromolecules, it should ultimately prove possible to detect interactions between substrate/analyte and individual (labelled) biological macromolecules immobilized in the apertures. The possibility of analyzing the dynamic behavior of single biomacromolecules operating in their natural environment is discussed.

Citation Download Citation

Robert J. G. Carr, Sumaia Al-Shukri, Elaine A. Perkins, Thierry Rodard, and Tony Atkinson "Submicron optical sources for single macromolecule detection", Proc. SPIE 1796, Chemical, Biochemical, and Environmental Fiber Sensors IV, (30 April 1993); https://doi.org/10.1117/12.143509

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available