New advances in 3D visualization of the cornea

Barry R. Masters

doi:10.1117/12.147516

24 June 1993 New advances in 3D visualization of the cornea

Barry R. Masters

Proceedings Volume 1877, Ophthalmic Technologies III; (1993) https://doi.org/10.1117/12.147516
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

This paper demonstrates the three-dimensional visualization of the ex vivo rabbit cornea. The cornea is an ideal test specimen to evaluate techniques for 3-D reconstruction and visualization of unstained, unfixed, transparent living tissues from a stack of optical sections. The 0.4 mm thick, transparent, specimen has been optically sectioned into 365 sections using a laser scanning confocal microscope with a water immersion objective. Isometric sampling resulted in near-cubic voxels which compensated for the reduced microscopic resolution in the z axis as compared to x and y resolution. This paper demonstrates the first three-dimensional visualization of the posterior stroma and the basal epithelium of the ex vivo rabbit cornea.

Citation Download Citation

Barry R. Masters "New advances in 3D visualization of the cornea", Proc. SPIE 1877, Ophthalmic Technologies III, (24 June 1993); https://doi.org/10.1117/12.147516

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available