DNA deformation in gel electrophoresis

Pascal Mayer; Jean Sturm; G. Weill

doi:10.1117/12.146161

17 June 1993 DNA deformation in gel electrophoresis

Pascal Mayer, Jean Sturm, G. Weill

Proceedings Volume 1922, Laser Study of Macroscopic Biosystems; (1993) https://doi.org/10.1117/12.146161
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

A quantitative study of the rise, magnitude, and decay of birefringence of DNA in agarose gel under an electric pulse E has been performed. It provides us with the molecular weight dependence of the characteristic times which describe the deformation of the chain during the transport and which are the parameters requested for designing electric pulse programs for pulsed field gel electrophoresis capable of achieving separation of DNA fragments of very high molecular weight.

Citation Download Citation

Pascal Mayer, Jean Sturm, and G. Weill "DNA deformation in gel electrophoresis", Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); https://doi.org/10.1117/12.146161

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