Laser experiments on DNA electrophoretic mobilities

Benjamin Chu; Evgeny V. Sokolov; A. R. Khokhlov

doi:10.1117/12.146218

17 June 1993 Laser experiments on DNA electrophoretic mobilities

Benjamin Chu, Evgeny V. Sokolov, A. R. Khokhlov

Proceedings Volume 1922, Laser Study of Macroscopic Biosystems; (1993) https://doi.org/10.1117/12.146218
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

By combining fluorescence recovery after photobleaching with gel electrophoresis, known as movements of fluorescence pattern after photobleaching (MOFPAP), it is possible to measure the time-dependent electrophoretic mobility of stained DNA fragments in a gel over relatively short time periods. The creation of a narrower stained DNA band by photobleaching can also shorten the running time and increase the resolution in a gel electrophoresis experiment for analytical purposes. In addition, a frequency analysis of MOFPAP signals has been simulated to demonstrate the potential capability of MOFPAP as an analytical tool for determining the presence of multiple species of DNA fragments in gel electrophoresis by measuring the electrophoretic mobility of each species present without spatial separation of different DNA fragments.

Citation Download Citation

Benjamin Chu, Evgeny V. Sokolov, and A. R. Khokhlov "Laser experiments on DNA electrophoretic mobilities", Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); https://doi.org/10.1117/12.146218

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