Electroporation visualized under a multishot pulsed laser fluorescence microscope system

Hiroyasu Itoh; Irene I. K. Yu; Masahiro Hibino; Tsuyoshi Hayakawa; Kazuhiko Kinosita Jr.

doi:10.1117/12.161357

11 October 1993 Electroporation visualized under a multishot pulsed laser fluorescence microscope system

Hiroyasu Itoh, Irene I. K. Yu, Masahiro Hibino, Tsuyoshi Hayakawa, Kazuhiko Kinosita Jr.

Proceedings Volume 2002, Ultrahigh- and High-Speed Photography, Videography, and Photonics '93; (1993) https://doi.org/10.1117/12.161357
Event: SPIE's 1993 International Symposium on Optics, Imaging, and Instrumentation, 1993, San Diego, CA, United States

Abstract

We describe a new fluorescence microscope system, which is the third generation of our pulsed-laser microscope systems developed for the purpose of capturing rapid cellular phenomena. Time resolution of this latest version is supported by the combination of a Q- switched Nd:YAG laser producing a burst of 4 pulses and a large format framing camera. We obtain series images at intervals on the order of 10 microsecond(s) with exposure times of 30 ns. With this multi-shot pulsed laser fluorescence microscope system, we examined the behavior of the transmembrane potential in a sea urchin egg under an intense electric field. Irreversible process of cell electroporation was revealed in serial images taken under a single electric pulse of microsecond duration.

Citation Download Citation

Hiroyasu Itoh, Irene I. K. Yu, Masahiro Hibino, Tsuyoshi Hayakawa, and Kazuhiko Kinosita Jr. "Electroporation visualized under a multishot pulsed laser fluorescence microscope system", Proc. SPIE 2002, Ultrahigh- and High-Speed Photography, Videography, and Photonics '93, (11 October 1993); https://doi.org/10.1117/12.161357

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