Fluorescence detection of single molecules using pulsed near-field optical excitation and time-correlated photon counting

W. Patrick Ambrose; Peter M. Goodwin; John C. Martin; Richard A. Keller

doi:10.1117/12.180866

21 July 1994 Fluorescence detection of single molecules using pulsed near-field optical excitation and time-correlated photon counting

W. Patrick Ambrose, Peter M. Goodwin, John C. Martin, Richard A. Keller

Author Affiliations +

Proceedings Volume 2125, Laser Techniques for Surface Science; (1994) https://doi.org/10.1117/12.180866
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Pulsed excitation, time correlated single photon counting, and time gated detection are used in near-field optical microscopy to enhance fluorescence images and measure the fluorescence lifetimes of single molecules of Rhodamine 6G on silica surfaces. Time gated detection is used to reject prompt scattered background and to improve the image signal to noise ratio. The excited state lifetime of a single Rhodamine 6G molecule is found to depend on the position of the near-field probe. We attribute the lifetime variations to spontaneous emission rate alterations by the fluorescence reflected from and quenching by the aluminum coated probe.

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W. Patrick Ambrose, Peter M. Goodwin, John C. Martin, and Richard A. Keller "Fluorescence detection of single molecules using pulsed near-field optical excitation and time-correlated photon counting", Proc. SPIE 2125, Laser Techniques for Surface Science, (21 July 1994); https://doi.org/10.1117/12.180866

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