Protein and immunohistochemical analysis after high-energy pulse-repetitive Nd:YAG laser impact on the rabbit lens

Victor V. Lazo; Helen P. Makarenkova; Ernest V. Boiko M.D.

doi:10.1117/12.182966

17 August 1994 Protein and immunohistochemical analysis after high-energy pulse-repetitive Nd:YAG laser impact on the rabbit lens

Victor V. Lazo, Helen P. Makarenkova, Ernest V. Boiko M.D.

Author Affiliations +

Proceedings Volume 2134, Laser-Tissue Interaction V; and Ultraviolet Radiation Hazards; (1994) https://doi.org/10.1117/12.182966
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

The purpose of the current study was to identify products of protein degradation and immunohistochemical changes of proteins distribution after high energy laser application. The rabbit lenses were irradiated in vivo with pulse-repetitive (F equals 50 Hz, (tau) _im equals 250 microsecond(s) , P_aver equals 10 W) 1.06 (mu) km wavelength laser radiation. Sodium dodecyl sulfate polyacrilamide electrophoresis of lens proteins following laser treatment showed decrease of 30 and 27 kDa molecular weight polypeptides in soluble fraction, loss of 70 kDa and decrease of 26 kDa molecular weight polypeptides in the insoluble fraction. New high molecular weight proteins in insoluble fraction were observed. Using the anti-MP70 monoclonal antibodies for immunofluorescence microscopy MP70 membrane junctional protein distribution after the laser cataract formation has been studied. Immunofluorescence experiments revealed an abundance of MP70 antigen in the normal lens outer cortex. No labeling after the laser impact in outer cortex and nucleus was found.

Citation Download Citation

Victor V. Lazo, Helen P. Makarenkova, and Ernest V. Boiko M.D. "Protein and immunohistochemical analysis after high-energy pulse-repetitive Nd:YAG laser impact on the rabbit lens", Proc. SPIE 2134, Laser-Tissue Interaction V; and Ultraviolet Radiation Hazards, (17 August 1994); https://doi.org/10.1117/12.182966

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