Three-dimensional imaging of biological specimens with standing wave fluorescence microscopy

Brent Bailey; Vijay Krishnamurthi; Daniel L. Farkas; D. Lansing Taylor; Frederick Lanni

doi:10.1117/12.172101

4 April 1994 Three-dimensional imaging of biological specimens with standing wave fluorescence microscopy

Brent Bailey, Vijay Krishnamurthi, Daniel L. Farkas, D. Lansing Taylor, Frederick Lanni

Proceedings Volume 2184, Three-Dimensional Microscopy: Image Acquisition and Processing; (1994) https://doi.org/10.1117/12.172101
Event: IS&T/SPIE 1994 International Symposium on Electronic Imaging: Science and Technology, 1994, San Jose, CA, United States

Abstract

Axial resolution in fluorescence microscopy can be improved significantly by using standing wave illumination to selectively excite planes within the depth of field of the microscope. When the specimen is thinner than 0.18 micrometers , an estimate of its 3D structure may be determined from three images within the same focal plane without re-focusing. Thicker objects require a combination of multi-focal-plane data and/or a priori knowledge.

Citation Download Citation

Brent Bailey, Vijay Krishnamurthi, Daniel L. Farkas, D. Lansing Taylor, and Frederick Lanni "Three-dimensional imaging of biological specimens with standing wave fluorescence microscopy", Proc. SPIE 2184, Three-Dimensional Microscopy: Image Acquisition and Processing, (4 April 1994); https://doi.org/10.1117/12.172101

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