Two photon excitation in time-resolved fluorescence microscopy

Pekka E. Hanninen; Stefan W. Hell; Ari Kuusisto; Lotta Lehtela; Juha Salo; Erkki J. Soini

doi:10.1117/12.172107

4 April 1994 Two-photon excitation in time-resolved fluorescence microscopy

Pekka E. Hanninen, Stefan W. Hell, Ari Kuusisto, Lotta Lehtela, Juha Salo, Erkki J. Soini

Proceedings Volume 2184, Three-Dimensional Microscopy: Image Acquisition and Processing; (1994) https://doi.org/10.1117/12.172107
Event: IS&T/SPIE 1994 International Symposium on Electronic Imaging: Science and Technology, 1994, San Jose, CA, United States

Abstract

Resonant two photon excitation of UV absorbing dyes with red light is a new concept in 3D microscopy. This technique provides functionality which is virtually equivalent to a confocal microscope. Two photon excitation allows excitation of UV dyes without special UV corrected optics. Resonant two photon excitation in microscopy, as known until now, utilizes expensive, high powered pulsed femto- or picosecond lasers. In this paper a cost effective alternative to resonant two photon is presented.

Citation Download Citation

Pekka E. Hanninen, Stefan W. Hell, Ari Kuusisto, Lotta Lehtela, Juha Salo, and Erkki J. Soini "Two-photon excitation in time-resolved fluorescence microscopy", Proc. SPIE 2184, Three-Dimensional Microscopy: Image Acquisition and Processing, (4 April 1994); https://doi.org/10.1117/12.172107

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