Fiber optical sampling of NADH concentration in guinea pig hearts during ischemia

Thomas Bocher; Juergen Beuthan; Olaf Minet; Isabella Schmitt; B. Fuchs; Gerhard J. Mueller

doi:10.1117/12.198717

4 January 1995 Fiber optical sampling of NADH concentration in guinea pig hearts during ischemia

Thomas Bocher, Juergen Beuthan, Olaf Minet, Isabella Schmitt, B. Fuchs, Gerhard J. Mueller

Proceedings Volume 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging; (1995) https://doi.org/10.1117/12.198717
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

A new method to detect and quantify ischemic states of tissue is presented. One important indicator of the metabolic and ischemic status of a cell is the concentration ratio of NADH : NAD⁺. The use of time-resolved laser- induced fluorescence techniques (LIF) in combination with previous biochemical measurements of NADH (Nicotineamide Adenine Dinucleotide) concentrations in tissue offer the possibility of quantitative NADH detection. The essential of the new method is a double-rescaling procedure of the fluorescence signal taking into account both the optical limitations of the device and the individually and locally varying optical tissue properties which are the scattering coefficient (mu) _s, the absorption coefficient (mu) _a and the anisotropy factor g. All the data presented in this paper were acquired from experiments with in vivo guinea pig hearts.

Citation Download Citation

Thomas Bocher, Juergen Beuthan, Olaf Minet, Isabella Schmitt, B. Fuchs, and Gerhard J. Mueller "Fiber optical sampling of NADH concentration in guinea pig hearts during ischemia", Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); https://doi.org/10.1117/12.198717

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