Paper
31 January 1995 Peculiarities of spectroscopic information of whole blood in atherosclerosis
Alphiya Ya. Khairullina, Tatiana V. Oleinik, Lira B. Yusupova, Natalia Prigoun
Author Affiliations +
Proceedings Volume 2326, Photon Transport in Highly Scattering Tissue; (1995) https://doi.org/10.1117/12.200845
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France
Abstract
The coefficient of diffuse reflection and light transmission measurements in an optically thick layer of blood at atherosclerosis conditions under multiple scattering of light in the visual and nearest IR-spectra region (590 -900 nm) were measured for calculation of the absorption coefficients of the material of particles and surrounding medium K((lambda) ) and parameter Q (the latter parameter was defined by the sizes of erythrocytes and aggregates and by refraction coefficient of red cells relative to plasma at atherosclerosis). For the main quantitative spectroscopy of particles the K1((lambda) ) for known value of K((lambda) ) and the parameter Q determinations it is necessary to have the knowledge of relative volume part H occupied by particles. In the case of a high concentration of particles H >= 0.2 as it takes place in the blood the parameters Q and K((lambda) ) are in dependence of H (H - is hematocrit ration for the case of whole blood). It should be noted that spectroscopy of multiple scattering light can give some information out of main absorption bands with the higher accuracy and higher light scattering. The latter value provides the opportunity of determination of faint absorption bands which couldn't be achieved by other methods. The method proposed is characterized by absence of probe preparations, approach to in viva conditions, expressivity, and high informativity of each experiment. A many-fold investigation of the blood of healthy men in the spectral region 650 - 810 nm shows the electron spectrum of absorption of molecular hemoglobin hem is the most optically active blood spectra component K((lambda) ). The broadening of spectral investigations, as in short wave or long wave areas of the spectrum, by the use of multiple scattering methods for calculations of K((lambda) ) and Q((lambda) ) enlarges the number of chromophores studied.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Alphiya Ya. Khairullina, Tatiana V. Oleinik, Lira B. Yusupova, and Natalia Prigoun "Peculiarities of spectroscopic information of whole blood in atherosclerosis", Proc. SPIE 2326, Photon Transport in Highly Scattering Tissue, (31 January 1995); https://doi.org/10.1117/12.200845
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KEYWORDS
Blood

Absorption

Ions

Multiple scattering

Spectroscopy

Particles

Plasma

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