Time-resolved spectroscopy of mitochondria, cells, and rat tissues under normal and pathological conditions

Bertrand Beauvoit; Toshiyuki Kitai; Hanli Liu; Britton Chance

doi:10.1117/12.200813

31 January 1995 Time-resolved spectroscopy of mitochondria, cells, and rat tissues under normal and pathological conditions

Bertrand Beauvoit, Toshiyuki Kitai, Hanli Liu, Britton Chance

Author Affiliations +

Proceedings Volume 2326, Photon Transport in Highly Scattering Tissue; (1995) https://doi.org/10.1117/12.200813
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

In this study, the detailed dependence of the light scattering on the tissue architecture and intracellular composition was investigated. The reduced scattering coefficient ((mu) _s') of isolated rat liver mitochondria, isolated liver cells and various rat tissues was measured at 780 nm by using time-resolved spectroscopy and a sample-substitution protocol. In a first part, extrapolations of the in vitro data to the in vivo situation showed that the mitochondrial compartment contributes for 73% of the scattering of the hepatocytes and about 100% of that of the whole liver. Finally, by analyzing different normal rat tissues and tumors, we have shown that the tissue (mu) _s' is independent on the cell concentration in the tissue but is roughly proportional to the tissue mitochondrial content.

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Bertrand Beauvoit, Toshiyuki Kitai, Hanli Liu, and Britton Chance "Time-resolved spectroscopy of mitochondria, cells, and rat tissues under normal and pathological conditions", Proc. SPIE 2326, Photon Transport in Highly Scattering Tissue, (31 January 1995); https://doi.org/10.1117/12.200813

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