Meso-tetrahydroxyphenylchlorin (mTHPC) fluorescence kinetics in mice

Laurent Morlet; Veronique Vonarx-Coinsmann; Marie-Therese Foultier; Thierry Patrice

doi:10.1117/12.203401

1 March 1995 Meso-tetrahydroxyphenylchlorin (mTHPC) fluorescence kinetics in mice

Laurent Morlet, Veronique Vonarx-Coinsmann, Marie-Therese Foultier, Thierry Patrice

Proceedings Volume 2371, 5th International Photodynamic Association Biennial Meeting; (1995) https://doi.org/10.1117/12.203401
Event: Fifth International Photodynamic Association Biennial Meeting, 1994, Amelia Island, FL, United States

Abstract

Analysis of sensitizer kinetics is essential for the performance of light irradiation when tumor concentration and tumor-to-normal tissue ratios are optimal. In our study, nude mice were grafted with human adenocarcinoma 15 days before m-THPC IP injection. Fluorescence was recorded through an optic fiber spectrofluorometer at 650, the most intense, and 714 nm, and intensity was proportional to the injected dose. In tumor, skin and muscle, maximum fluorescence was obtained with 1.6 mg/kg 72 h after injection (44 counts per second). Tumor- to-skin and -muscle ratios obtained by HPLC analysis and spectrofluorometric measurements decreased between 12 and 72 h (15 to 1.5), indicating that tumor selectivity decreases with time.

Citation Download Citation

Laurent Morlet, Veronique Vonarx-Coinsmann, Marie-Therese Foultier, and Thierry Patrice "Meso-tetrahydroxyphenylchlorin (mTHPC) fluorescence kinetics in mice", Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); https://doi.org/10.1117/12.203401

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