Usefulness of a wavelength tunable optical parametric oscillator laser on photodynamic therapy

Yoshiro Nishiwaki; Takato O. Yoshida; Satoshi Nakamura; Shozo Baba; Eiji Matsusawa; Hideo Suzuki; Toru Hirano

doi:10.1117/12.203387

1 March 1995 Usefulness of a wavelength tunable optical parametric oscillator laser on photodynamic therapy

Yoshiro Nishiwaki, Takato O. Yoshida, Satoshi Nakamura, Shozo Baba, Eiji Matsusawa, Hideo Suzuki, Toru Hirano

Proceedings Volume 2371, 5th International Photodynamic Association Biennial Meeting; (1995) https://doi.org/10.1117/12.203387
Event: Fifth International Photodynamic Association Biennial Meeting, 1994, Amelia Island, FL, United States

Abstract

By rotating the optical axis of a nonlinear optical crystal ((beta) -BaB₂O₄), a tunable laser beam could be obtained from an optical parametric oscillator (OPO) laser. When the crystal was optically pumped by the third harmonics of the 1064 nm Nd:YAG laser, we had a coherent beam from 410 nm through 2550 nm continuously without changing the optical cavity. We compared photodynamic therapy (PDT) effects of two photosensitizers, phenophorbide a(Phd) and Photosan-3(Ph-3, hematoporphyrin-polyester), on Wistar rat liver. Twenty-four hours after sensitization (5 mg/kg i.v.), 670 nm and 630 nm light (75 mW/cm²) was irradiated for Phd and Ph-3 respectively at energy doses of 25, 50, and 100 J/cm². The rats were sacrificed 24 hours after laser irradiation and analyzed pathologically. Phd produced more severe necrosis than Ph-3. Twenty-five J/cm² of Phd was identical with 100 J/cm² of Ph-3. Next, we treated HeLa cell tumors of nude mice by Phd 670 nm PDT and Ph-3 630 nm PDT. The PDT effects of the two photosensitizers on HeLa cell tumors were similar to those on normal liver tissue. In conclusion the OPO laser could make it possible to compare PDT effects of photosensitizers by activating them with their matched wavelengths.

Citation Download Citation

Yoshiro Nishiwaki, Takato O. Yoshida, Satoshi Nakamura, Shozo Baba, Eiji Matsusawa, Hideo Suzuki, and Toru Hirano "Usefulness of a wavelength tunable optical parametric oscillator laser on photodynamic therapy", Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); https://doi.org/10.1117/12.203387

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