Light scattering and fluorescence of single particles measured by a scanning flow cytometer

Valerii Pavlovich Maltsev; Andrej V. Chernyshev; Alexander A. Doroshkin; Erkki J. Soini

doi:10.1117/12.206019

3 April 1995 Light scattering and fluorescence of single particles measured by a scanning flow cytometer

Valerii Pavlovich Maltsev, Andrej V. Chernyshev, Alexander A. Doroshkin, Erkki J. Soini

Proceedings Volume 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics; (1995) https://doi.org/10.1117/12.206019
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

A novel type of Scanning Flow Cytometer (SFC) has been developed for improving particle analysis from light scattering data. The use of the SFC and the Flying Light Scattering Indicatrix (FLSI) method allows absolute measurement of the size and refractive index of spherical particles. The separation efficiency of the single particle analysis using FLSI method is verified measuring the size distribution of standard latex particles. It is shown that particles differed in size more than 1.2 micrometers can be separated by the FLSI method to different classes. In the second part of this paper the feasibility of a Pulsed Laser Flow Cytometer (PLFC) equipped by a pulsed nitrogen laser is tested for fluorometric analysis of human lymphocytes stained with FITC-labeled monoclonal antibodies. In addition the feasibility for the fluorescence measurement in time-resolved regime of the PLFC is analyzed from measurement of photon collection efficiency of scanning optical system.

Citation Download Citation

Valerii Pavlovich Maltsev, Andrej V. Chernyshev, Alexander A. Doroshkin, and Erkki J. Soini "Light scattering and fluorescence of single particles measured by a scanning flow cytometer", Proc. SPIE 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, (3 April 1995); https://doi.org/10.1117/12.206019

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