Optical melting as a tool for optimizing sequencing by hybridization (SBH) analysis of DNA

Mitchel J. Doktycz; K. Bruce Jacobson; K. L. Beattie; Robert S. Foote

doi:10.1117/12.206038

3 April 1995 Optical melting as a tool for optimizing sequencing by hybridization (SBH) analysis of DNA

Mitchel J. Doktycz, K. Bruce Jacobson, K. L. Beattie, Robert S. Foote

Proceedings Volume 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics; (1995) https://doi.org/10.1117/12.206038
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Sequencing by hybridization is a technique that relies on the specific hybridization properties of nucleic acids to derive sequence information. Hybridization properties are highly dependent on the DNA sequence and the solution environment. Identification of the optimal SBH conditions can be obtained by optical melting. By optical melting of 128 octamer pairs that have the appropriate choice of nucleic acid structures, a useful model of stability has been obtained which will aid in the design and implementation of SBH assays.

Citation Download Citation

Mitchel J. Doktycz, K. Bruce Jacobson, K. L. Beattie, and Robert S. Foote "Optical melting as a tool for optimizing sequencing by hybridization (SBH) analysis of DNA", Proc. SPIE 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, (3 April 1995); https://doi.org/10.1117/12.206038

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