Use of diode laser energy (808 nm) for selective photothermolysis of contaminated wounds

Kenneth Eugene Bartels D.V.M.; Rebecca J. Morton; D. Thomas Dickey; Ernest L. Stair D.V.M.; Marie E. Payne; Steven A. Schafer; Robert E. Nordquist

doi:10.1117/12.209149

12 May 1995 Use of diode laser energy (808 nm) for selective photothermolysis of contaminated wounds

Kenneth Eugene Bartels D.V.M., Rebecca J. Morton, D. Thomas Dickey, Ernest L. Stair D.V.M., Marie E. Payne, Steven A. Schafer, Robert E. Nordquist

Author Affiliations +

Proceedings Volume 2395, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems V; (1995) https://doi.org/10.1117/12.209149
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

The objective of this study was to compare the antibacterial effects of selective laser treatments on contaminated wounds. Comparisons were made using the diode (808 nm) laser with and without an accompanying chromophore, Nd:YAG (1064 nm), and KTP (532 nm) lasers. A known quantity (mean concentration of 7.9 +/- 1.9 CFU/ml X 10⁸) of bacterial organisms (Staphylococcus intermedius) was inoculated into skin incisions aseptically made on the backs of experimental rabbits. The bacteria were allowed to adhere/colonize for one hour, and each site was then treated with various laser modalities. Tissue samples were taken at one and forty-eight hours after treatment. The number of viable bacteria was then quantitated (CFU/ml) by a spot-plate counting technique. Initial results illustrated the fact that selective photothermolysis of superficial cellular layers of targeted tissue occurred with an accompanying decrease of bacterial numbers that virtually created a `clean' (organisms numbers less than 10⁵ per gram of tissue) wound. This effect was especially noticeable using the diode laser (808 nm) and an accompanying chromophore, indocyanine green.

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Kenneth Eugene Bartels D.V.M., Rebecca J. Morton, D. Thomas Dickey, Ernest L. Stair D.V.M., Marie E. Payne, Steven A. Schafer, and Robert E. Nordquist "Use of diode laser energy (808 nm) for selective photothermolysis of contaminated wounds", Proc. SPIE 2395, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems V, (12 May 1995); https://doi.org/10.1117/12.209149

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