Approaches from a single- to a multiparameter evanescent wave sensor

Daniel Bock; Andreas Hartmann; Sepp Kaul; Michael Martin; V. Schulz; Juergen M. Wolfrum; Stefan Seeger

doi:10.1117/12.229506

8 January 1996 Approaches from a single- to a multiparameter evanescent wave sensor

Daniel Bock, Andreas Hartmann, Sepp Kaul, Michael Martin, V. Schulz, Juergen M. Wolfrum, Stefan Seeger

Author Affiliations +

Proceedings Volume 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology; (1996) https://doi.org/10.1117/12.229506
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

Evanescent wave sensors are an interesting tool for the fast detection of reactions between biomolecules. By using small and inexpensive diode lasers together with fluorescent dyes in the red spectral region, it was possible to construct a highly specific sensor. This was due to the use of the immobilization of receptor molecules via the Langmuir-Blodgett technique which enhances the specificity drastically. As a clinically relevant system an immunoassay of the tumor marker mucine was investigated. Mucine could be detected with a sandwich test using the antibody system BM-2/BM-7. In order to detect several analytes at one time, there are two possible ways: time-resolved detection using multiplex-dyes and the parallelization of the sensor by using several fibers simultaneously. A position-resolved evanescent wave sensor using a CCD camera is described.

Citation Download Citation

Daniel Bock, Andreas Hartmann, Sepp Kaul, Michael Martin, V. Schulz, Juergen M. Wolfrum, and Stefan Seeger "Approaches from a single- to a multiparameter evanescent wave sensor", Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); https://doi.org/10.1117/12.229506

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