Surface second-harmonic generation (SSHG): a new scheme for immunoassay

Liqun Yang; Daniel McStay; Peter J. Quinn

doi:10.1117/12.238808

24 April 1996 Surface second-harmonic generation (SSHG): a new scheme for immunoassay

Liqun Yang, Daniel McStay, Peter J. Quinn

Proceedings Volume 2676, Biomedical Sensing, Imaging, and Tracking Technologies I; (1996) https://doi.org/10.1117/12.238808
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

A new optical immunoassay scheme based on surface second harmonic generation (SHG) is proposed. The utility of the technique has been investigated by deposition of sample antigen and antibody (bovine serum albumin & mouse anti-bovine serum albumin IgG antibody) on glass surfaces. The laser-induced second harmonic signals generated from these sample surfaces were monitored using a photomultiplier tube (Thorn EMI, 9524A). The surface second harmonic generation characteristics of an enzyme-linked immunoassay process were investigated, which is parallely monitored by a conventional ELISA method. The SSHG immunoassay experiment was performed using a conventional sandwich immunoassay scheme on ELISA immunoassay plates. The laser-induced surface-second harmonic signals generated from sample wells of different populations of the antigen-antibody complex were subsequently measured using the photomultiplier tube. To verify the new SSHG immunoassay results, a conventional ELISA sandwich immunoassay was performed, and their results compared. The new SSHG immunoassay results showed a virtually identical conclusion as that of conventional ELISA sandwich immunoassay. The SSHG readings are promotional to that of the ELISA data. However, the readings from the new SSHG immunoassay were found much larger, which is in agreement with the theoretical expectation. In this paper, detailed experimental results of the new SSHG immunoassay are described, and subsequently compared with those of a conventional ELISA immunoassay. The feasibility and advantages of the SSHG as a new immunoassay technique also are discussed.

Citation Download Citation

Liqun Yang, Daniel McStay, and Peter J. Quinn "Surface second-harmonic generation (SSHG): a new scheme for immunoassay", Proc. SPIE 2676, Biomedical Sensing, Imaging, and Tracking Technologies I, (24 April 1996); https://doi.org/10.1117/12.238808

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