System for fast measurement of in-vivo tissue fluorescence spectra under multiple wavelength excitations

Haishan Zeng; Calum E. MacAulay; Branko Palcic

doi:10.1117/12.238815

24 April 1996 System for fast measurement of in-vivo tissue fluorescence spectra under multiple wavelength excitations

Haishan Zeng, Calum E. MacAulay, Branko Palcic

Proceedings Volume 2676, Biomedical Sensing, Imaging, and Tracking Technologies I; (1996) https://doi.org/10.1117/12.238815
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Tissue autofluorescence properties are excitation wavelength dependent. In this work, we developed a nitrogen dye laser -- OMA system capable of quickly measuring tissue autofluorescence spectra in vivo under multiple wavelength excitation. The system consists of a nitrogen dye laser with stepper motor for wavelength selection, an OMA with gated intensified detector, and a PC computer. A bifurcated fiber optic bundle is used to conduct the excitation laser light and to collect the fluorescence light. Gating electronics are used to achieve a high signal to noise ratio, allowing fluorescence measurements to be performed with ambient light on or under white light illumination during endoscopy. Excitation wavelength changes are performed automatically and quickly by the stepper motor which is controlled by the OMA PIA port. The system has been tested with a tissue phantom. The time needed to switch from one excitation wavelength to the next excitation wavelength is about one second.

Citation Download Citation

Haishan Zeng, Calum E. MacAulay, and Branko Palcic "System for fast measurement of in-vivo tissue fluorescence spectra under multiple wavelength excitations", Proc. SPIE 2676, Biomedical Sensing, Imaging, and Tracking Technologies I, (24 April 1996); https://doi.org/10.1117/12.238815

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