Multicolor FISH using a novel spectral bioimaging system

Dirk G. Soenksen; Yuval Garini; Irit Bar-Am

doi:10.1117/12.239550

10 May 1996 Multicolor FISH using a novel spectral bioimaging system

Dirk G. Soenksen, Yuval Garini, Irit Bar-Am

Proceedings Volume 2678, Optical Diagnostics of Living Cells and Biofluids; (1996) https://doi.org/10.1117/12.239550
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

FISH (fluorescence in situ hybridization) is a cytogenetic technique for locating and quantifying small genetic defects that cannot be detected by traditional karyotyping, the banding analysis of stained chromosomes. The selectivity of FISH holds the promise for the accurate, low cost and fast diagnosis of genetic disorders, birth defects, and various types of cancer that are not detectable by other means. To aid investigators with their FISH measurements, filter-based digital imaging systems are available to enhance and analyze faint FISH images. The accuracy and reliability of traditional FISH measurements is generally compromised when there is (1) sample autofluorescence, (2) image movement, e.g., due to lack of registration between images acquired through different filter sets, or (3) spectral overlap between fluorescent DNA probe emission spectra. SpectraCube^TM is an interferometric imaging method which is not subject to the limitations of filters-based systems. By measuring a definitive spectrum, simultaneously at all points in a sample, SpectraCube^TM has the potential to revolutionize FISH by enabling the detection and separation of a large number of spectrally and spatially overlapping fluorescent DNA probes, and by eliminating sample autofluorescence. Details of the Applied Spectral Imaging SD200 spectral bio-imaging system and the results of a six-color FISH measurement will be discussed.

Citation Download Citation

Dirk G. Soenksen, Yuval Garini, and Irit Bar-Am "Multicolor FISH using a novel spectral bioimaging system", Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); https://doi.org/10.1117/12.239550

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available