Peptide sequencing using MALDI on time-of-flight and ion trap mass spectrometers

Robert J. Cotter; Timothy J. Cornish; Marcela Cordero; Vladimir M. Doroshenko

doi:10.1117/12.237625

1 April 1996 Peptide sequencing using MALDI on time-of-flight and ion trap mass spectrometers

Robert J. Cotter, Timothy J. Cornish, Marcela Cordero, Vladimir M. Doroshenko

Proceedings Volume 2680, Ultrasensitive Biochemical Diagnostics; (1996) https://doi.org/10.1117/12.237625
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Two instruments have been developed in our laboratory for the direct amino-acid sequencing of peptides. The first is a time-of-flight (TOF) mass spectrometer that utilizes a coaxial, curved-field reflectron which provides simultaneous focusing of product ins formed by post- source decay (PSD) and obviates the need for scanning or stepping the reflectron voltage. The second is a matrix-assisted laser desorption/ionization (MALDI) ion trap mass spectrometer (ITMS). In this instrument, MALDI ions are trapped efficiently by ramping the trapping rf field, and a linear mass scale is achieved by modulating the axial excitation voltage during mass scanning. Monoisotopic mass selection and excitation is accomplished using phase- modulated SWIFT (stored waveform inverse Fourier transform) techniques, while efficient CID (collision-induced dissociation) performance is achieved using pulsed introduction of Xe and other heavier gases. Both mass spectrometers have been designed as compact instruments for sequencing peptides in partially fractionated mixtures.

Citation Download Citation

Robert J. Cotter, Timothy J. Cornish, Marcela Cordero, and Vladimir M. Doroshenko "Peptide sequencing using MALDI on time-of-flight and ion trap mass spectrometers", Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); https://doi.org/10.1117/12.237625

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