Polarized scattered light as a probe for structure and change in bioparticles

William S. Bickel; Mary E. Stafford

doi:10.1117/12.237598

1 April 1996 Polarized scattered light as a probe for structure and change in bioparticles

William S. Bickel, Mary E. Stafford

Proceedings Volume 2680, Ultrasensitive Biochemical Diagnostics; (1996) https://doi.org/10.1117/12.237598
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Polarized light scattered from small particles -- spheres, fibers, dust, defects and biomaterial -- carries information about the optical and geometrical properties and even more esoteric properties of the scatterer. This highly developed, powerful, but under used technique is sensitive enough to detect a 0.5 nanometer radius change in a 1.0 micron diameter quartz fiber. The complete set of 16 polarized light scattering signals from any scatterer form a 16- element Mueller matrix, S_ij, which is the signature of the scatterer's status quo. Changes in cell morphology, internal arrangement and metabolic activity cause changes in radius, refractive index and absorption and therefore changes in the polarization signals S_ij which act as the probe. We demonstrate how the S_ij can type human red blood cells, do bacteria autopsies, distinguish between different types of white blood cells, bacteria, spores, and study cell life cycle and degradation as biomaterial undergoes biologically significant change.

Citation Download Citation

William S. Bickel and Mary E. Stafford "Polarized scattered light as a probe for structure and change in bioparticles", Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); https://doi.org/10.1117/12.237598

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available