Real-time system for fluorescence lifetime imaging

Rinaldo Cubeddu; Gianfranco L. Canti; Antonio Pifferi; Paola Taroni; Gianluca Valentini

doi:10.1117/12.275518

16 June 1997 Real-time system for fluorescence lifetime imaging

Rinaldo Cubeddu, Gianfranco L. Canti, Antonio Pifferi, Paola Taroni, Gianluca Valentini

Proceedings Volume 2976, Biomedical Sensing, Imaging, and Tracking Technologies II; (1997) https://doi.org/10.1117/12.275518
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

In this paper we report on the experimental setup and the performances of a fluorescence lifetime imaging system designed by the authors for cancer detection. The experimental apparatus relies on a CCD video camera coupled to a light intensifier and on a pulsed laser. Two or more fluorescence images are acquired within suitable time windows at different delays, with respect to the excitation pulses. The images are processed using matrix algorithms implemented in the fast processor of an advanced frame grabber; then, the resulting lifetime map is displayed in pseudocolor or gray shade images. The instrumentation is fully computer controlled and the whole process takes about 1 second per lifetime image. Using a faster processor this time could be easily reduced to some milliseconds for true real time measurements. The system has been successfully applied to detect experimental tumors in mice sensitized with hematoporphyrin derivative, which is a clinically approved photosensitizing drug.

Citation Download Citation

Rinaldo Cubeddu, Gianfranco L. Canti, Antonio Pifferi, Paola Taroni, and Gianluca Valentini "Real-time system for fluorescence lifetime imaging", Proc. SPIE 2976, Biomedical Sensing, Imaging, and Tracking Technologies II, (16 June 1997); https://doi.org/10.1117/12.275518

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