Image reconstruction for three-dimensional transmitted-light DIC microscopy

Chrysanthe Preza; Donald L. Snyder; Jose-Angel Conchello

doi:10.1117/12.271264

10 April 1997 Image reconstruction for three-dimensional transmitted-light DIC microscopy

Chrysanthe Preza, Donald L. Snyder, Jose-Angel Conchello

Proceedings Volume 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV; (1997) https://doi.org/10.1117/12.271264
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

The derivation of an iterative method for phase reconstruction from differential-interference-contrast (DIC) images is presented here. Because DIC imaging is direction sensitive, in our approach we estimate a specimen's phase function using multiple DIC images obtained by rotating the specimen. Results obtained from testing the method via two- dimensional simulations demonstrate that the use of multiple DIC images at different specimen rotations yield phase reconstructions that more closely resemble the phase function of phantoms than the unprocessed DIC images. Improvement in resolution was also achieved: two points separated by half the Rayleigh resolution limit for coherent illumination not resolved in the unprocessed DIC image, were successfully resolved in the reconstructed phase images. Our results show that phase reconstructions are quantitatively better and resolution is improved when two or more DIC images are used in the reconstruction.

Citation Download Citation

Chrysanthe Preza, Donald L. Snyder, and Jose-Angel Conchello "Image reconstruction for three-dimensional transmitted-light DIC microscopy", Proc. SPIE 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV, (10 April 1997); https://doi.org/10.1117/12.271264

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