Enhanced fluorescence microscope and its application

Susheng Wang; Qin Li; Xin Yu

doi:10.1117/12.294540

12 December 1997 Enhanced fluorescence microscope and its application

Susheng Wang, Qin Li, Xin Yu

Proceedings Volume 3173, Ultrahigh- and High-Speed Photography and Image-based Motion Measurement; (1997) https://doi.org/10.1117/12.294540
Event: Optical Science, Engineering and Instrumentation '97, 1997, San Diego, CA, United States

Abstract

A high gain fluorescence microscope is developed to meet the needs in medical and biological research. By the help of an image intensifier with luminance gain of 4 by 10⁴ the sensitivity of the system can achieve 10^-6 1x level and be 10⁴ times higher than ordinary fluorescence microscope. Ultra-weak fluorescence image can be detected by it. The concentration of fluorescent label and emitting light intensity of the system are decreased as much as possible, therefore, the natural environment of the detected call can be kept. The CCD image acquisition set-up controlled by computer obtains the quantitative data of each point according to the gray scale. The relation between luminous intensity and output of CCD is obtained by using a wide range weak photometry. So the system not only shows the image of ultra-weak fluorescence distribution but also gives the intensity of fluorescence of each point. Using this system, we obtained the images of distribution of hypocrellin A (HA) in Hela cell, the images of Hela cell being protected by antioxidant reagent Vit. E, SF and BHT. The images show that the digitized ultra-sensitive fluorescence microscope is a useful tool for medical and biological research.

Citation Download Citation

Susheng Wang, Qin Li, and Xin Yu "Enhanced fluorescence microscope and its application", Proc. SPIE 3173, Ultrahigh- and High-Speed Photography and Image-based Motion Measurement, (12 December 1997); https://doi.org/10.1117/12.294540

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KEYWORDS

Luminescence

Microscopes

Biological research

Charge-coupled devices

Data acquisition

Environmental sensing

Fluorescent markers

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